Figure 5

Relative basal expression of IL-8, IL-6, ICAM-1 and IκBα genes in HeLa-IE63 cells compared to control cells. mRNA levels (IL-8, IL-6, ICAM-1, and IκBα) were determined by quantitative real-time PCR in unstimulated HeLa cells expressing or not IE63 and normalized using the β2-microglobuline transcripts. Experiments were done at least in triplicate. Differences (n-fold) between samples were calculated using the standard-curve method and the 2^-ΔCt method. ρ-values were calculated using the graphpad quickcalcs software [59] : **, not significantly different from control (Inv ; p-value ≥ 0.05).