Table 3 Detection yield, copy number and cycle threshold obtained in qPCR for the commercial RT systems SuperScript III and PowerScript

3.75

6.30

2.90

2.49

4.07

20.37

12.37

10 ± 5

16 ± 20

7 ± 3

6 ± 5

10 ± 1

52 ± 28

32 ± 6

(36.61 ± 1.44)

(37.87 ± 1.13)

(36.65 ± 0.6)

(37.19 ± 1.21)

(36.06 ± 0.2)

(33.89 ± 0.98)

(34.41 ± 0.2)

4.48

6.81

7.82

13.78

20.11

36.31

34.48

11 503 ± 6 190

17479 ± 1 861

20 053 ± 1 168

35 342 ± 4 588

51 592 ± 2 574

93 150 ± 10 206

88 458 ± 6 161

(26.28 ± 0.99)

(25.51 ± 0.11)

(25.21 ± 0.09)

(24.37 ± 0.2)

(23.8 ± 0.07)

(22.93 ± 0.16)

(23 ± 0.01)

0 ± 0

26 ± 3

99 ± 12

142 ± 24

229 ± 31

1 653 ± N/A

5 314 ± 1 090

(40 ± 0)

(33.27 ± 0.29)

(30.98 ± 0.16)

(30.35 ± 0.29)

(29.55 ± 0.22)

(26.22 ± N/A)

(24.29 ± 0.37)

1.21

3.97

5.12

3.61

3.61

17.67

8.23

3 ± 1

10 ± 5

13 ± 3

9 ± 3

11 ± 5

45 ± 18

21 ± 5

(38.34 ± 0.47)

(36.48 ± 0.9)

(35.99 ± 0.41)

(36.56 ± 0.58)

(36.35 ± 0.76)

(34.17 ± 0.75)

(35.27 ± 0.37)

9.29

12.89

23.99

18.69

27.28

54.55

59.43

23 837 ± 723

33 078 ± 4 291

61 560 ± 340320

47 944 ± 9 523

69 982 ± 20 743

139 950 ± 10 479

152 480 ± 9 523

(6.01 ± 0.05)

(25.53 ± 0.2)

(24.72 ± 0.77)

(23.2 ± 0.09)

(24.42 ± 0.45)

(23.33 ± 0.12)

(23.2 ± 0.09)

2 ± 1

31 ± 3

168 ± 16

35 ± 8

58 ± 40

2 440 ± 650

6 118 ± 308

(36.85 ± 1.26)

(32.47 ± 0.18)

(30 ± 0.11)

(32.29 ± 0.35)

(31.9 ± 1.39)

(26.07 ± 0.38)

(24.69 ± 0.07)