An unusual S-adenosylmethionine synthetase gene from dinoflagellate is methylated

BMC Molecular Biology

Table 2 Inhibition of DNA methylation of AdoMet synthetase by 5-azacytidine and L-ethionine

	The degree of digestibility of CcAdoMets gene		Inhibition of DNA methylation of CcAdoMets gene
	HpaII	MspI
Untreated	*	****	/
5-azacytidine treatment	*****	*****	*****
L-ethionine treatment	***	****	***

* Represents the degree of digestibility and inhibition of DNA methylation
Southern blotting showing the restriction pattern of C. cohnii DNA by HpaII and MspI without any treatment; after treating with 200 μM 5Aza and after treating with 1.2 mM L-Eth. Higher the digestibility of CcAdoMetS gene by HpaII indicated higher degree of inhibition of DNA methylation.

ISSN: 1471-2199