Figure 2

(A) Multiple alignments of AdoMet synthetase proteins. The deduced amino acid sequences of AdoMet synthetase proteins were aligned with Plasmodium falciparum (AAG13449), Leishmania infantum (AAB88448), Saccharomyces cerevisiae (P10659), Oryza sativa (CAA81481) and Homo sapiens (NP_000420). The identical amino acids were marked with Asterisk (*); Three conversed motifs are bracketed; The unique sequences and the flexible loops in CcAdoMet synthetase protein of C. cohnii are shaded. (B) Diagram showing the hypothetical positions of the three extra loops of CcAdoMetS on the basis of E. coli AdoMetS 3D model. The 3D model of the E. coli enzyme was generated form PDB file of the enzyme (Protein Data Bank ID: 1MXA) using RasMol program. The amino and carboxylic termini are labeled N and C, respectively. The three loops (Loops A, B & C) are marked with shaded eclipses on the right. The marked amino acid residues show the insertion points of the loops and are in E. coli enzyme numbering. The PKC site marked the equivalent position in rat enzyme for phosphorylation regulation. The mobile invisible loop is arbitrarily drawn as a blue line at the lower-left corner. Entrances of substrate binding sites are marked with yellow arrows.