Figure 2

cDNA-based Element patterns of the 185/333 genes. A. Exon element patterns. Gene sequences were manually aligned based on alignments of cDNA sequences. The insertion of gaps (black lines), which defined the 25 elements (shown as colored boxes) [30]. A consensus sequence containing all the elements and the locations of the two exons is shown at the top. The source of each gDNA is indicated by the colored dots in the columns labeled "Animal". Blue dots indicate that the pattern was isolated from animal 2, red dots from animal 4, and green dots from animal 10. The frequency with which the patterns were found is indicated in the column labeled "Freq". The element patterns are sorted into groups (grey background shading) based on the subtype of Ex15 (specified by the letter in the box). The locations of the five types of repeats [27,30] are shown at the bottom of A (red boxes indicate type 1 repeats; blue = type 2; green = type 3; yellow = type 4; and purple = type 5). Patterns not previously identified from cDNA analysis [27,30] are denoted with an asterisk (*). The position of the stop codon in element Ex25 is indicated by the letter present in the box and the size/shape of the box (Additional file 2, Additional file 3). There are 31 unique patterns based on both exon elements and intron type. B: Intronless genes with unique element patterns amplified from BAC DNA. Untranslated sequence (due to a missense deletion) is indicated by lighter shading and narrower boxes for E2.7. The locations of the repeats are the same as A.