Table 1 Primer sets used to determine the gene structure of TaASY1
From: Expression and functional analysis of TaASY1 during meiosis of bread wheat (Triticum aestivum)
Region | Sense Sequence | Antisense Sequence | Product size |
|---|---|---|---|
Exon 1–3 | CCACGCGCGCACACAACACA | GCAATCCGGAGCAAATTCCT | 2.2 Kb |
Exon 2–3 | GCTTGCTTGCTCGGTTCCATTTG | CCAACCAATAATCCTGCAATGTATTTG | 1.0 Kb |
Exon 3–9 | GGTGTCTATGATGCCTTACAAAAG | CTTGCTATTGACATTCCCCAC | 1.6 Kb |
Exon 10-Intron 14 | GCTAATGATGCTAACAGTGATGATGAC | GTGGACTAACACTATAAAGAAATCTG | 0.8 Kb |
Exon 7–17 | CGCTAGTTTCACTTATGAGGACCTTG | CCATCAAGCTTGCCCTGAAG | 1.9 Kb |
Exon 16-Intron 20 | GGAAGAGGTTGCCATGCACAAT | CCAAACCCAATGTGACAGAGGTAAG | 1.2 Kb |
Exon 16–22 | GGAAGAGGTTGCCATGCACAAT | GTTGCGCCGAGGCTCCTTGC | 1.8 Kb |