Figure 4

TaASY1 transcript expression levels observed from northern analysis, microarray and Q-PCR technology platforms. (A) Tissue specific northern analysis of TaASY1 expression in seedling (S), young leaf (YL), mature leaf (ML), root tips (RT) and meiotic tissue (MT). (B) Meiosis specific northern analysis of TaASY1 expression during pre-meiosis (PM), leptotene to pachytene (LP), diplotene to anaphase I (DA), telophase I to telophase II (TT), tetrads (T) and immature pollen (IP). For both analyses, ethidium bromide stained rRNA was used as a loading control. (C) Using the Affymetrix GeneChip^® Wheat Genome Array, two probes were identified that matched TaASY1: Ta.9186.1.S1_at (1591 bp to 1771 bp – solid circles) and Ta.9186.2.S1_at (111 bp to 586 bp – open circles). While expression is considerably higher in Ta.9186.1.S1, it is evident that as meiosis concludes, transcript levels are minimal (as seen in both probes). PM, LP, DA, TT, T, IP as in (B) with the addition of mature anthers (MAN). (D) Tissue-specific and meiosis staged Q-PCR analysis of TaASY1. Expression levels detected were minimal in leaf, root and mature anthers when compared to the meiosis staged tissues investigated. PM, LP, DA, TT, T, IP, MAN as in (C) with the addition of root tips (RT) and leaf (L). Y axis is expressed as normalised mRNA copies/μL.