Figure 3From: Biochemical characterization of a recombinant Japanese encephalitis virus RNA-dependent RNA polymeraseOptimization of JEV RdRp assay conditions. Effect of temperature (A), pH (B), K+ ion (C), and Mn2+ ion (D) on JEV RdRp activity. RdRp assays were performed with the poly(A)/(U)20 template under the indicated conditions. The RdRp activity was measured as in Figure 2 and is presented as the percentage of that observed under each optimal condition. Shown is the mean and standard error from three independent experiments.Back to article page