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Table 3 Candidate reference genes for normalization and their expression stability values calculated by NormFinder

From: In search of suitable reference genes for gene expression studies of human renal cell carcinoma by real-time PCR

Ranking order

Gene

Stability value

1

PPIA

0.074

2

TBP

0.123

3

ACTB

0.199

4

HMBS

0.301

5

SDHA

0.308

6

HPRT1

0.402

7

GAPDH

0.436

8

RPLPO

0.453

9

ALAS1

0.481

10

TUBB

0.514

  1. A low stability value as an estimate of the combined intra- and intergroup variation of the respective gene corresponds to a high expression stability of the respective gene between the matched malignant and non-malignant samples.
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BMC Molecular Biology

ISSN: 1471-2199

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