Figure 5From: Down-regulation of human topoisomerase IIα expression correlates with relative amounts of specificity factors Sp1 and Sp3 bound at proximal and distal promoter regionsSp3 transcriptional repression is dominant over activation by Sp1. All transfections were performed in triplicate with 1.0 μg of -617 wt topoisomerase IIα reporter plasmid and 1 μg of pCMV Sport β-galactosidase control vector. Relative luciferase activity was calculated by arbitrarily setting the control (no added Sp1 or Sp3) to 100%. Results are expressed as ± SEM where n = 9, *** p < 0.001, ** p < 0.01, and * p < 0.05Back to article page