Figure 3From: Down-regulation of human topoisomerase IIα expression correlates with relative amounts of specificity factors Sp1 and Sp3 bound at proximal and distal promoter regionsSp1 and Sp3 are antagonistic at the topoisomerase IIα promoter. All transfections were performed in triplicate with 1.0 μg of -617 wt topoisomerase IIα reporter plasmid and 1 μg of pCMV Sport β-galactosidase control vector. Relative luciferase activity was calculated by arbitrarily setting the control (0 μg, containing only topoisomerase IIα reporter plasmid and pCMV Sport β-galactosidase control vector) to 100%. Results are expressed as ± SEM where n = 9, *** p < 0.001, ** p < 0.01, and * p < 0.05.Back to article page