Table 1 Sequences of antisense oligonucleotides (ASOs) employed in the experiments
ASO name | Sequence (5'-3') ACGU = 2'O-methyl RNA bases, acgu = RNA bases |
|---|---|
skip 27-5 | AUAAAAGCUCCAUACUGAAG |
skip 27-3 | CAAAACUGUAUAGGAGAGAU |
skip 27–53 | AUAAAAGCUCCAUACUGAAGCAAAACUGUAUAGGAGAGAU |
skip 27-5B | AUAAAAGCUCCAUACUGAAGAUUUUAUUAGAUUCAUAACA |
skip 27-3B | CUGUAUAGGAGAGAUUUUGUAUUUUAUUAGAUUCAUAACA |
skip 27-3B scram | CAAGUAGAUAUUAUAUGUUGUUACUUGUAUAUAUCGAAUG |
skip 27-B | AUUUUAUUAGAUUCAUAACA |
skip 27–53 A1 | AUAAAAGCUCCAUACUGAAGCAAAACUGUAUAGGAGAGAUucgaguaugauagggacuuagggug |
skip 27–53 mut A1 | AUAAAAGCUCCAUACUGAAGCAAAACUGUAUAGGAGAGAUucgaguaugauacgcacuuacgcug |
skip 27-3B A1 | CUGUAUAGGAGAGAUUUUGUAUUUUAUUAGAUUCAUAACAucgaguaugauagggacuuagggug |
skip 27-3B mut A1 | CUGUAUAGGAGAGAUUUUGUAUUUUAUUAGAUUCAUAACAucgaguaugauacgcacuuacgcug |
skip 27-ex 6–25 | UCUUCGAUUUUGUGUGUUCC |
skip 27-ex 31–50 | UUUAGUCUUAGAGGCUAACG |
skip 27-ex 61–80 | UGCACUGAAGUCACGGUGUG |
skip 27-ex 88–107 | UUCAUAUUUGCCAUCUUCUU |
skip 27-ex 6–25 scram | CUGUUUGUUUCUGACGCUUU |
skip 27-ex 31–50 scram | CCAUGGGUCUUUUAGUAAAG |
skip 27-ex 61–80 scram | UGGACAUCGCAGACGUUGGU |
skip 27-ex 88–107 scram | GUUCUCUUUUUCAUUCCAUA |
FLuc A1 | GUCUGCUCGAAGCGGCCGGCucgaguaugauagggacuuagggug |