From: Selection of reliable reference genes for qPCR studies on chondroprotective action
Experimental condition | mRNA relative to untreated control group (mean ± SD) | |||||
---|---|---|---|---|---|---|
 | ACTB | GAPDH | B2M | HPRT1 | SDHA | YWHAZ |
IL-1β | 0.93 ± 0.16 | 0.86 ± 0.10 | 1.06 ± 0.17 | 0.97 ± 0.15 | 0.91 ± 0.11 | 0.92 ± 0.13 |
Simultaneous incubation with curcumin [c]1 | 0.72 ± 0.07 | 0.74 ± 0.11 | 0.85 ± 0.10 | 0.88 ± 0.11 | 0.76 ± 0.10 | 0.78 ± 0.08 |
Simultaneous incubation with curcumin [c]2 | 0.41 ± 0.04* | 0.89 ± 0.16 | 0.64 ± 0.06 | 0.86 ± 0.08 | 0.61 ± 0.06 | 0.85 ± 0.11 |
Pre-incubation with curcumin [c]1 | 0.87 ± 0.13 | 0.98 ± 0.15 | 0.94 ± 0.18 | 0.97 ± 0.11 | 0.95 ± 0.23 | 1.02 ± 0.21 |
Pre-incubation with curcumin [c]2 | 0.37 ± 0.06* | 0.46 ± 0.05* | 0.28 ± 0.04* | 0.38 ± 0.04* | 0.09 ± 0.02* | 0.65 ± 0.06 |
Curcumin only | 0.28 ± 0.01* | 0.48 ± 0.06* | 0.31 ± 0.03* | 0.38 ± 0.05* | 0.10 ± 0.01* | 0.63 ± 0.04 |