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Table 4 Relative mRNA levels of C-28/I2 chondrocytes under the influence of IL-1β and curcumin.

From: Selection of reliable reference genes for qPCR studies on chondroprotective action

Experimental condition

mRNA relative to untreated control group (mean ± SD)

 

ACTB

GAPDH

B2M

HPRT1

SDHA

YWHAZ

IL-1β

0.93 ± 0.16

0.86 ± 0.10

1.06 ± 0.17

0.97 ± 0.15

0.91 ± 0.11

0.92 ± 0.13

Simultaneous incubation with curcumin [c]1

0.72 ± 0.07

0.74 ± 0.11

0.85 ± 0.10

0.88 ± 0.11

0.76 ± 0.10

0.78 ± 0.08

Simultaneous incubation with curcumin [c]2

0.41 ± 0.04*

0.89 ± 0.16

0.64 ± 0.06

0.86 ± 0.08

0.61 ± 0.06

0.85 ± 0.11

Pre-incubation with curcumin [c]1

0.87 ± 0.13

0.98 ± 0.15

0.94 ± 0.18

0.97 ± 0.11

0.95 ± 0.23

1.02 ± 0.21

Pre-incubation with curcumin [c]2

0.37 ± 0.06*

0.46 ± 0.05*

0.28 ± 0.04*

0.38 ± 0.04*

0.09 ± 0.02*

0.65 ± 0.06

Curcumin only

0.28 ± 0.01*

0.48 ± 0.06*

0.31 ± 0.03*

0.38 ± 0.05*

0.10 ± 0.01*

0.63 ± 0.04

  1. Boldfaced values p < 0.05
  2. * p < 0.05 and change > 2-fold
  3. mRNA levels under the treatment with curcumin in different experimental conditions are presented relative to the untreated control group calculated by the equation E-ΔCt (Mean ± SD, n = 9; value of 1 represents average level of mRNA expression in untreated control group). [c]1, low concentration of curcumin (5 μM); [c]2, high concentration of curcumin (50 μM).