Figure 4

Re-replication of regions adjacent to telomeres. Probe values were plotted as a function of their distance from the closest telomere for chromosomes 1 and 2 in fission yeast or for all chromosomes in budding yeast (E). Chromosome 3, which has atypical telomeres due to the presence of rDNA repeats, was omitted from the analysis. Probe values for wild-type (A; green), cds1 Δ (B; blue), rad3 Δ (C; red), and all three strains (D; green, blue, and red) induced for Cdc18* over-expression at 17, 21, and 25 hours of Cdc18* induction are shown. Re-replication is enhanced up to 50 kb from the ends of telomeres in wild-type and cds1 Δ cells and up to 100 kb in rad3 Δ cells. (E)Re-replication of sub-telomeric regions up to 50 kb from the ends of chromosomes was also enhanced in checkpoint-competent S. cerevisiae cells as shown in this figure from Tanny et al. The figure shows relative enrichment for each spot on their microarray plotted as a function of its distance to the closest telomere for both the re-replicating strain (black) and wild-type strain (gray) [14].