Table 1 Amplification efficiency determined by various methods of real-time PCR data analysis. Arithmetical means ± SD and coefficients of variation (in parentheses) are shown.
Gene/Method | Standard curve | DART-PCR | LinRegPCR | Liu&Saint-exp |
|---|---|---|---|---|
IL-1β | 0.998 | 1.033 ± 0.061 (5.95) | 0.950 ± 0.074 (7.76) | 0.974 ± 0.033 (3.42) |
IL-6 | 0.900 | 0.912 ± 0.046 (5.05) | 0.881 ± 0.075 (8.51) | 0.870 ± 0.029 (3.35) |
TNF-α | 1.012 | 1.033 ± 0.024 (2.32) | 0.974 ± 0.072 (7.45) | 0.991 ± 0.032 (3.32) |
GM-CSF | 0.918 | 0.978 ± 0.044 (4.49) | 0.932 ± 0.063 (6.75) | 0.898 ± 0.013 (1.44) |
ACTB | 0.981 | 1.086 ± 0.046 (4.21) | 0.999 ± 0.031 (3.10) | 0.978 ± 0.044 (4.53) |
SDHA | 0.967 | 1.072 ± 0.052 (4.88) | 0.980 ± 0.064 (6.55) | 1.017 ± 0.044 (4.27) |
HPRT | 1.022 | 1.069 ± 0.064 (5.95) | 0.978 ± 0.109 (11.1) | 0.976 ± 0.048 (4.49) |