Figure 3

Identification of full-length MID1 transcripts. (A) 5'UTR exons, coding region and 3'UTR of MID1. Arrows indicate location of primers. (B-D) Ethidium bromide stained gels of RT-PCR products obtained using RNA from fibroblasts (B), testis (C) or fetal brain (D). Asterisks indicate products that could not be sequenced due to their low abundance. RT-PCR products obtained with combinations of primer sets 1–3, with primers located in 5'UTR exons 1c, v1a or 1e. (E, F) Fetal tissue Northern blots hybridized with probes corresponding to 5'UTR exons 1c (E) or 1e (F). Arrows indicate MID1 transcripts.