Table 3 Bacterial strains and plasmids used

Restriction deficient mutant of C. glutamicum ATCC* 13032, Δ (cglIM-cglIR-cglIIR), Nx^r

[47]

RES167 with sugR deletion, after double crossover with pLMJ1, Nx^r

This work

RES167 with cg2118 deletion, after double crossover with pLMJ2, Nx^r

This work

RES167 with cg2118/sugR double-deletion, after double crossover with pLMJ1 and pLMJ2, Nx^r

This work

F-ë-fhuA 2 [lon] ompT lacZ::T7 gene1 gal sulA 11 Δ(mcrC-mrr)114::IS10 R(mcr-73::miniTn10-TetS)2 R(zgb-210::Tn10)(TetS) endA 1 [dcm]

New England Biolabs

recA 1, endA 1, gyrA 96, thi, hsdR 17, supE 44, relA 1, Δ(lac-proAB)/F' [traD 36, proAB⁺, lacI^q, lacZ ΔM15]

Takara Bio Inc.

JM109 with expression vector pLGI1 for plasmid isolation, Ap^r

This work

ER2566 with expression vector pLGI1 for the overexpression of SugR, Ap^r

This work

mobilizable E. coli cloning vector, allows for double crossover in C. glutamicum, sacB, lacZ α, Km^r

[50]

E. coli vector, lac promoter, lacZ α, ccdB lethal gene, Km^r

Invitrogen

E. coli expression vector, C-terminal intein tag, T7 promoter, lacI, rrnB T1, Ap^r

New England Biolabs

pK18mobsacB containing 588 bp sugR deletion fragment (sugR-d1/4), obtained by Eco RI-Bam HI fusion, Km^r

This work

pK18mobsacB containing 1117 bp cg2118 deletion fragment (cg2118-d1/4), obtained by Bgl II-Eco RI fusion, Km^r

This work

pTYB1 containing sugR (777 bp), obtained by NdeI-SapI fusion, Ap^r

This work