Figure 6

Constructs containing trimers of mTARC STAT6-binding sites are induced to varying degrees by IL-4. RAW 267.4 cells were transfected as described in Figure 4A with 5 μg luciferase-conjugated mTARC STAT6 site trimers and 3 μg STAT6 expression vector. E denotes the Emr1 minimal promoter and T1-11 denote mTARC putative STAT6 sites within the constructs. F refers to the forward orientation of the promoter. Data are representative of at least 2 transfections and results are displayed as fold induction of sample relative luciferase activity (luciferase/β-galactosidase) with cytokine treatment +/- SD.