Figure 2

Somatic polyadenylation cassettes are preferred tomale germ cell-specific cassettes. A) At the top is a map indicating the positions of the various polyadenylation sites on the cyclin A2 mRNA. Above the diagram is the positions of the poly(A) sites used [27], and below is the name of the cassette. The diagram is approximately drawn to scale. Below, ATCC 3T3 cells (mouse embryonic fibroblasts) were transfected with plasmids containing somatic or male germ cell-specific cassettes indicated and extracts were assayed for activity as described in Figure 1. B) Same as A, but using CREM and c-abl cassettes. The poly(A) positions at the top are from [26, 28]. For both A and B, asterisks indicate significantly different from the respective somatic cassette as determined by Student's T-test (p < 0.01).