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Figure 3 | BMC Molecular Biology

Figure 3

From: Plasma membrane calcium ATPase (PMCA4): A housekeeper for RT-PCR relative quantification of polytopic membrane proteins

Figure 3

Comparison of reference gene expression in drug-resistant cell lines. The two parental cell lines, MCF-7 and KB-3-1, and several of their drug-resistant subclones were evaluated using RT-PCR. The drug-resistant MCF-7 cell lines were as follows: MCF-7/ADR (doxorubicin-treated)[18]; MCF-7FL-1000 (flavopiridol-treated)[20]; MCF-7/VP16 (etoposide-treated) [19]; and MCF-7-ADRVP (adriamycin- and verapamil-treated)[21]. The KB cell lines were KB-A1 (doxorubicin-treated); KB-V1 (vinblastine-selected) and KB-C1 and KB-8.5-11 (colchicine-selected)[22]. A) ATP1A1; B) PMCA4; C) GAPDH; and D) HPRT1. The error bars represent standard deviation (n = 4).

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BMC Molecular Biology

ISSN: 1471-2199

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