Figure 5

Average growth rates for sequence libraries with defined distances between Cbf1 and Met31/32 binding sites. We generated libraries of pooled transformants containing single-copy plasmids with the indicated distances between Cbf1 and Met31/32 binding sites. We induced reporter gene expression by transferring these pooled cultures into media lacking histidine, leucine and methionine, plus the indicated concentration of 3-AT (see Methods for details). The growth rate, expressed in doublings per hour, for each pooled culture was obtained from regular measurements of OD₆₀₀. The average growth rate and standard error of the mean are plotted for three independent trials.