| dpm |
---|
70 S (CAG1632) | 4507 ± 379 |
70 S (XL-1B) | 3688 ± 345 |
50 S | 1241 ± 97 |
30 S | 1274 ± 141 |
poly(U) | 541 ± 36 |
70 S + poly(U) | 2511 ± 239 |
- | 179 ± 34 |
- [35S]RNase III (30 000 dpm) was incubated with 1 A260 unit 70 S ribosomes or with the same amount of isolated subunits followed by separation of ribosomes from unbound radioactivity by a Sephacryl S400 spin column using NH4Cl concentration as indicated. Ribosome-bound RNase III was determined by TCA precipitation and counting the precipitate for radioactivity. The results are shown in dpm. In the control experiments 2 A260 units of poly(U) was added to the incubation mixture. The control with [35S]RNase III alone is also shown. The data presented here are average results of 4 parallel experiments.