Figure 4

Analysis of a human COX-2 promoter region. (A) Scheme of the promoter region. Transcription factor binding sites were identified using the computer program Factor (HUSAR program package, DKFZ Heidelberg). Note the presence of two NF-κB binding sites NF-κB₁ (promoter-distal) and NF-κB₂ (promoter-proximal). (B) Analysis of COX-2 promoter activity. Two PCR-generated COX-2 promoter constructs in the promoter-less luciferase vector pGL-2 (Promega, Heidelberg) were choosen for transfection in HEK 293 cells. The luciferase activity of the empty vector pGL-2 was set to one. The fold induction of luciferase activity is depicted in a linear scale on the ordinate. Both COX-2 promoter constructs show a more than 300-fold activity over the promoter-less vector pGL-2. The standard deviation of triplicate transfection assays is depicted as arrow bars.