A novel, non-radioactive eukaryotic in vitro transcription assay for sensitive quantification of RNA polymerase II activity

Table 3 Effect of TATA-binding protein on the transcription efficiency

Template	Reaction	Signal (RLU average)	% RNA synthesis*	% RNA synthesis
Template	Reaction	Signal (RLU average)	% RNA synthesis*	TATA mutant vs. standard**
HS-DNA_std	Positive	1306740	100% ± 3%	100% ± 3%
	Negative	9768
	α-amanitin 200 nM	161585	12% ± 1%
HS-DNA_mut	Positive	985650	100% ± 10%	75% ± 7%
	Negative	11795
	α-amanitin 200 nM	130255	12% ± 1%

Transcription reactions were performed using the standard DNA template (HS-DNA_std) or a TATA-box mutated template (HS-DNA_mut). Transcription efficacy was determined by QuantiGene quantification.
*Compared to respective average positive control, less the respective negative controls.
**Compared to average positive control on standard template, less the respective negative controls.

ISSN: 1471-2199