Figure 1From: Egr-1 regulates the transcription of NGX6 gene through a Sp1/Egr-1 overlapping site in the promoterIdentification of NGX6 minimal promoter (-86/+100). (A) Schematic illustration of deletion constructs of NGX6 proximal promoter pGL3-159/+276. (B) Luciferase activity of the deleted constructs in COS7 and HT-29 cells. pGL3-control, pGL3-enhancer and NGX6 promoter constructs were transfected into COS7 and HT-29 cells, respectively. All of the vectors were cotransfected with β-galactosidase vector for normalizing transfection efficiency. Luciferase activities were analyzed 48 hours post transfection. Data are expressed as mean ± SE of three independent experiments.Back to article page