Figure 4

Effect of miR-378 overexpression on C2C12 bone transcription program: upregulation. A) Venn diagram representing the number of probes on the microarray that are significantly (as defined in Methods) higher expressed in C2C12-pMirn378 cells than in control C2C12-pMirn0 cells at each time point during differentiation in the presence of 300 ng/ml BMP2. B) Enriched (p < 0.01) GO terms (in biological processes category) within the set of probes that are significantly higher expressed in C2C12-pMirn378 versus C2C12-pMirn0 cells at at least two consecutive time points during differentiation in the presence of 300 ng/ml BMP2. C) Microarray profiles of bone markers Sp7 transcription factor 7 (Sp7; 1418425_at), distal-less homeobox 5 (Dlx5; 1449863_a_at), alkaline phosphatase (Alpl; 1423611_at), vitamin D receptor (Vdr; 1418175_at), collagen type I alpha 1 (Col1a1; 1423669_at), platelet derived growth factor receptor alpha polypeptide (Pdgfra; 1421917_at), fibroblast growth factor receptor 3 (Fgfr3; 1421841_at) and Kazal-type serine peptidase inhibitor domain 1 (Kazald1; 1436528_at) at indicated time points during differentiation of C2C12-pMirn0 (light bullets) and C2C12-pMirn378 (dark bullets) cells treated with (squares) or without (circles) 300 ng/ml BMP2 as revealed from microarray analysis. Mean expression values +/− SD from triplicate microarray experiments are shown for all data points. When the error bar is not visible, the SD falls within the printed data point. All SD values are, however, listed in Additional file 2. AEV = average expression value; *significantly different expression in C2C12-pMirn378 compared to control C2C12-pMirn0 at the same time point and treatment (see Methods for definition).