Predicted gene product | Primer sequence | GC (%) | Tm (°C)a | Amplicon (bp) | Ex-Ex Spanningb | Efficiency (%) | R2 |
---|---|---|---|---|---|---|---|
Cycloisomerase | F: TCATAGGTGGCGTCAAGAACGTGT | 50.0 | 60.3 | 127 | No | 99.5 | 0.995 |
 | R: TCAGCTTGTCGTGCCGAAATTGTG | 50.0 | 60.3 |  |  |  |  |
SLC6 | F: TCGGACACTACGGAGACTACG | 57.1 | 57.1 | 73 | No | 104.2 | 0.997 |
 | R: ACCAAGGTGAAGGCGACG | 61.1 | 58.0 |  |  |  |  |
SLC6 Ex-Ex | F: CATGCACGATACTGTCTATTTCG | 43.5 | 53.6 | 122 | Yes | 100.0 | 0.998 |
 | R: CGTCCAACCAAAATAAGCCAGC | 50.0 | 57.0 |  |  |  |  |
Aldo-keto reductase | F: GAATGGGCTACGGAGAGACG | 60.0 | 57.3 | 114 | No | 99.5 | 0.998 |
 | R: GTACAGGCGTGAATTTGGTAGC | 50.0 | 56.2 |  |  |  |  |
sHSP | F: GACGAAGGATTCATCACCGTCG | 54.5 | 57.7 | 141 | No | 102.9 | 0.998 |
 | R: GACACCGTTGTCGAGGGTAG | 60.0 | 57.4 |  |  |  |  |
PEPCK | F: GCATTGCTCTGCAAACGTCG | 55.0 | 57.7 | 107 | No | 100.0 | 0.997 |
 | R: CAATCAAGGCTCGGTGAGGATC | 54.5 | 57.7 |  |  |  |  |
GDH | F: CAATGCCATCAACGCCATCAAGGA | 50.0 | 60.2 | 128 | No | 98.4 | 0.998 |
 | R: CAAAGCCGAGGTTGGCAAGAGTTT | 50.0 | 60.3 |  |  |  |  |
PFK | F: CGAGGTGGCATCCAAACGATTGC | 56.5 | 61.1 | 84 | No | 105.1 | 0.998 |
 | R: GCAGCCTGTGTATTGGTATCGTCG | 54.1 | 59.7 |  |  |  |  |
PFK Ex-Ex | F: GGAGAAAATCCGCTCGAGGTG | 57.1 | 57.9 | 111 | Yes | 99.4 | 0.997 |
 | R: CTTTGAGAGAACCGCAGCCTG | 57.1 | 58.4 |  |  |  |  |
FCP | F: CGTCTCATACCACGGCAC | 61.1 | 55.7 | 184 | No | 97.8 | 0.997 |
 | R: CTTGGATTGATGGTCCACGAG | 52.3 | 55.6 |  |  |  |  |