Table 1 Oligonucleotide primers used in this study
Name | Sequence | Purpose used |
|---|---|---|
AKI-F-S1 | ATGGCCTGCGGAGCGACGTT | ORF amplification |
AKI-F-A1 | TCAGGAGACATAACTAGCAGGCCG | ORF amplification |
β-actin-s1 | GCTGTGCTGTCCCTGTA | RT-PCR |
β-actin-a1 | GAGTAGCCACGCTCTGTC | RT-PCR |
GAP-S1 | CAACGGCGACACTCACTCCTC | RT-PCR |
GAP-A1 | TCGCAGACACGGTTGCTGTAG | RT-PCR |
TUBA-S1 | TGACATCACAAACGCCTGCTTC | RT-PCR |
TUBA-A1 | GCACCACATCTCCACGGTACAG | RT-PCR |
AKI-R-S1 | AGGACCAGCCCTCGTTCACACT | RT-PCR |
AKI-R-A1 | TCCGTATCTTCGCATGATCTGGT | RT-PCR |
HEPN-R-S1 | TACAAGGACAATGGTGGGGG | RT-PCR |
HEPN-R-A1 | GGCAAGGGCTGAGATGGAG | RT-PCR |
C1q-R-S1 | CTCCAGAAAACGAAGCAGGC | RT-PCR |
C1q-R-A1 | TGTCGCACATCATCAAGTGAAC | RT-PCR |
AKI-F-A7 | GCGTCGACGGAGACATAACTAGCAG | Plasmid construction |
AKI-T-A1 | GCGGTACCGGAGACATAACTAGCAG | Plasmid construction |
AD-AKI-S1 | GTGAATTCATGGCCTGCGGAGCGACGTT | Plasmid construction |
AD-AKI-A1 | CAGCTCGAGTCAGGAGACATAACTAGCAG | Plasmid construction |
C1q-S1 | GCCGAATTCAAGGGGGCACCAGGTCTTAA | Plasmid construction |
C1q-A1 | CAGGTCGACTCAGGCCGTGGGGAAGACGA | Plasmid construction |
HEPN-S3 | GTGAATTCGGCACGAGGCTCAGGTGGCA | Plasmid construction |
HEPN-A2 | CAGCTCGAGAGCCTCCTCTTTGTTTGGCC | Plasmid construction |
HEPN-A3 | CAGCTCGAGGGAAGGTTGACCGTGCCTTT | Plasmid construction |
HEPN-S5 | AGTGAATTCTCTGGCAGCTCCATCTCAGC | Plasmid construction |
HEPN-A4 | CAGCTCGAGCTATTTCACATATGCCTCAAC | Plasmid construction |
HEPN-ET-S1 | CCGAATTCAGGCTCAGGTGGCATCCT | Plasmid construction |
HEPN-ET-A1 | GTGCTCGAGCTATTTCACATATGCCTCA | Plasmid construction |