Figure 4
From: Clock-controlled mir-142-3p can target its activator, Bmal1
The upstream regulatory sequence of mir-142 gene contains a CLOCK-binding E-box. (A) A schematic representation of the conserved E-box element in the upstream regulatory sequence of the mouse mir-142 gene (obtained from UCSC Genome Browser Database). The arrow indicates the transcriptional orientation. Sequence alignments of mouse, rat, human, dog and cow (bottom) are shown. (B) Chromatin immunoprecipitation (ChIP) assays of NIH3T3 cells with ChIP-grade CLOCK antibody. The primers for amplifying the Per1 E-box or the upstream regulatory sequence of mir-20a gene were used as the positive and negative controls, respectively (left). The ChIP results were also quantified by quantitative real-time PCR (mean ± SD, n = 3). *P < 0.05 and **P < 0.01.