Figure 5
From: Increased complexity of Tmem16a/Anoctamin 1 transcript alternative splicing
Exon 18 deletion truncates the TMEM16A protein. A. Cartoon indicating the Tmem16a variant as a result of exon 18 exclusion identified in the transcript scanning assay. RT-PCR with a specific primer set (spanning the boundary of exons 17 and 19) verified the exclusion of exon 18 in mouse heart. B. Exon 18 exclusion truncates TMEM16A protein to 596 amino acids and the predicted hydropathy profile is shown. Red boxes represent the four transmembrane spanning domains, blue and pink lines represent intracellular and extracellular locations respectively.