Figure 3
From: TEAD1-dependent expression of the FoxO3a gene in mouse skeletal muscle
EMSA analysis of TEAD1 binding site in the FoxO3a promoter. Biotin-labeled oligonucleotide probes for the TEAD1 FoxO3a binding site were incubated with mouse skeletal muscle extracts. Competition experiments were performed using a 100-fold excess of mutant or unlabeled TEAD1 FoxO3a probes. Supershift assays were performed with anti-TEAD1 antibody. Arrows indicate the resulting bandshifts (FOX-W: normal probe; FOX-T: mutant probe; FOX-WB: unlabeled probe).