Figure 5

RAM product time-course analysis. Figure 5A: Capillary electrophoresis densitometry data with a simulated a gel-electrophoresis lane. RAM product peak numbers and capillary electrophoresis markers (M) are indicated. RFU, relative fluorescence units. Figure 5B. Per-peak DNA concentration vs. peak number (as defined in Figure 5A) is plotted for four RAM reaction time-points; reaction times (minutes) are indicated above each panel. Four independent determinations made for each time-point are shown as circles. Diamond symbols contain centered horizontal lines marking the mean for each peak and time point; the top and bottom points of the diamond mark the 95% confidence interval. Slopes determined by least-squares regression of the combined data for each time point are shown for each data group within the panels. A broken reference line showing slope -1 passes through the peak 1 data group for each time point. Figure 5C. Slope determinations are plotted for samples throughout the time course. Slopes were measured as in Figure 5B; each point represents an independent measurement.