From: Essential role for ALCAM gene silencing in megakaryocytic differentiation of K562 cells
ALCAM Luc reporter constructs | Forward | Reverse |
-1200 | 5'-AAATCACCGCTTAACTCAAAG-3' | 5'-CCTCCTCCTTCTTGGTGG-3' |
-1000 | 5'-CCGCGCTTCAACCACCTGCT-3' | |
-800 | 5'-CAGAAAGTGTTAGTCCCAGG-3' | |
-650 | 5'-CCGCGCTTCAACCACCTGCT-3' | |
-400 | 5'-CCGCCTCCTGCGAGTCCTTC-3' | |
-200 | 5'-GTTGACCGGGAGGGAGGAGG-3' | |
EMSA probes | Sense strand | |
- 850 GATA-1 wt | 5'-GCCAGAGGCCTTATCACTGG-3' | |
- 850 GATA-1 mt | 5'-GCCAGAGGCCTGCTCACTGG-3' | |
ChIP assay | Forward | Reverse |
GATA-1 | 5'-GGGTGGAGGGAGAGGGCAGTC-3' | 5'-AAGCAAGCACAGGAGCCGCGC-3' |
RT-PCR | Forward | Reverse |
ALCAM | 5'-AGTCTT CAT TATCAGGATGC-3' | 5'-GGGATCAGTTTTCTTTGTCA-3' |
γ-globin | 5'-ACTCGCTTCTGGAACGTCTGA-3' | 5'-GTATCTGGAGGACAGGGCACT-3' |
Glycophorin A | 5'-GGAATTCCAGCTCATGATCTCAGGATG-3' | 5'-TCCACATTTGGTTTGGTGAACAGAT3' |
Cmpl | 5'-GCACTGTGATGCTTTATGCAAC-3' | 5'-TGAACGGTTTAGAGGATGAGGA-3' |
CD44 | 5'-GATCCACCCCAATTCCATCTGTGC-3' | 5'-AACCGCGAGAATCAAAGCCAAGGC-3' |
CD61 | 5'-TATAGCATTGGACGGAAGGC-3' | 5'-GACCTCATTGTTGAGGCAGG-3' |
GAPDH | 5'-ATGGGGAAGGTGAAGGTCGG-3' | 5'-GACGGTGCCATGGAATTTGC-3' |
PKCα | 5'-CGAGGAAGGAAACATGGAACTCAG-3' | 5'-CCTGTCGGCAAGCATCACCTTT-3' |
PKCε | 5'-TCAATGGCCTTCTTAAGATCAAAA-3' | 5'-CCTGAGAGATCGATGATCACATAC-3' |
PKCθ | 5'-CTCGTCAAAGAGTATGTCGAATCA-3' | 5'-AATTCATTCAGTCCTTTGTGTCACTC-3' |
PKCδ | 5'-GCATCGCCTTCAACTCCTATGAGCT-3' | 5'-ACACACCCACGGTCACCTCAGA-3' |