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Table 8 Most stably expressed probe sets annotated for popular normalizing genes

From: Microarray analysis of relative gene expression stability for selection of internal reference genes in the rhesus macaque brain

Cycle study: brain region

HRT: brain region

rank/544

stability value

annotation target

probe set ID

exp

rank/1444

stability value

annotation target

probe set ID

exp

76

0.109

RPS27A

200017_at

++++

6

0.074

RPS27A

MmugDNA.43194.1.S1_at

-

182

0.141

RPL11

200010_at

+++

23

0.082

RPL13A

MmugDNA.16774.1.S1_s_at

++++

216

0.151

ALG9

228817_at

+

62

0.091

RPL32

MmugDNA.25831.1.S1_s_at

++++

217

0.152

RPL19

200029_at

+++

187

0.112

LDHA

MmugDNA.40348.1.S1_at

-

233

0.159

ARHGDIA

201168_x_at

++

350

0.135

ACTB

AFFX-Mmu-actin-3_s_at

++++

236

0.160

ACTB

200801_x_at

++++

382

0.140

GAPDH

AFFX-Mmu-gapdh-3_x_at

++++

261

0.169

RPS18

201049_s_at

++++

383

0.140

PGK1

MmugDNA.17381.1.S1_at

-

281

0.178

RPL13A

200716_x_at

++++

535

0.161

RPL19

MmugDNA.25770.1.S1_at

+++

333

0.201

GAPDH

212581_x_at

++++

628

0.182

ARHGDIA

MmugDNA.2575.1.S1_at

-

352

0.211

HSPCB

200064_at

++++

825

0.231

HSPCB

MmugDNA.32274.1.S1_at

+++

363

0.220

RPL32

200674_s_at

++++

842

0.235

RPS18

MmugDNA.43260.1.S1_at

++++

389

0.240

PGK1

200737_at

++

934

0.262

ALG9

MmugDNA.30425.1.S1_at

+

454

0.293

NONO

200057_s_at

+++

1129

0.329

NONO

MmugDNA.18264.1.S1_at

++

456

0.294

ALDOA

200966_x_at

+++

1271

0.410

RPL11

MmugDNA.25225.1.S1_at

+

464

0.302

LDHA

200650_s_at

++

     

Cycle study: cycle phase

HRT: treatment

rank/544

stability value

annotation target

probe set ID

exp

rank/1444

stability value

annotation target

probe set ID

exp

41

0.039

RPS27A

200017_at

++++

2

0.054

RPS27A

MmugDNA.43194.1.S1_at

-

87

0.045

RPL11

200010_at

+++

25

0.066

RPL32

MmugDNA.25831.1.S1_s_at

++++

110

0.048

RPL19

200029_at

+++

143

0.078

GAPDH

AFFX-Mmu-gapdh-3_x_at

++++

123

0.049

ACTB

200801_x_at

++++

164

0.079

RPL13A

MmugDNA.16774.1.S1_s_at

++++

150

0.051

ARHGDIA

201168_x_at

++

215

0.082

ACTB

AFFX-Mmu-actin-3_s_at

++++

182

0.053

RPL13A

200716_x_at

++++

294

0.086

PGK1

MmugDNA.17381.1.S1_at

-

204

0.056

RPS18

201049_s_at

++++

350

0.089

RPL19

MmugDNA.25770.1.S1_at

+++

253

0.060

ALG9

228817_at

+

463

0.095

ARHGDIA

MmugDNA.2575.1.S1_at

-

338

0.068

GAPDH

212581_x_at

++++

568

0.099

RPS18

MmugDNA.43260.1.S1_at

++++

347

0.069

HSPCB

200064_at

++++

571

0.099

HSPCB

MmugDNA.34686.1.S1_at

+++

361

0.073

RPL32

200674_s_at

++++

607

0.102

LDHA

MmugDNA.8511.1.S1_at

-

436

0.090

ALDOA

200966_x_at

+++

668

0.105

ALG9

MmugDNA.30425.1.S1_at

+

437

0.090

NONO

200057_s_at

+++

1212

0.168

RPL11

MmugDNA.25225.1.S1_at

+

478

0.104

LDHA

200650_s_at

++

1346

0.218

NONO

MmugDNA.770.1.S1_at

+

492

0.112

PGK1

200737_at

++

     
  1. Probe sets are listed according to rank. Higher rank indicates greater expression stability. Denominator in the rank column indicates the number of probe sets analyzed using criteria described in methods. Note that selection was narrowed to the 544 probe sets likely to be most representative of genes of interest where menstruating macaques were examined using the human GeneChip®. All 1444 probe sets that were annotated for genes of interest on the rhesus GeneChip® are represented in the table.
  2. "exp" = gene expression represented by hybridization signal intensity [52].
  3. "+" and "-" symbols represent signal intensity values averaged across all animals and obtained using Affymetrix® GCOS analysis, and global scaling to an average target intensity of 200. (-) = "undetectable", (+) = "signal intensity < 500", (++) = "signal intensity 500-1500", (+++) = "signal intensity 1501-10000". (++++) = "signal intensity > 10000"
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BMC Molecular Biology

ISSN: 1471-2199

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