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Table 5 20 most stably expressed probe sets detected in MAS 5.0 analysis of HRT study

From: Microarray analysis of relative gene expression stability for selection of internal reference genes in the rhesus macaque brain

HRT: brain region

rank/824

stability value

descriptor "GEN = "

gene symbol

probe set ID

exp

1

0.065

SUI1

---

MmugDNA.22897.1.S1_s_at

+++

2

0.072

B4GALT3

LOC719920

MmugDNA.396.1.S1_at

+

3

0.086

RPL13A

LOC698713

MmugDNA.16774.1.S1_s_at

++++

4

0.086

LDHB

LDHB

MmugDNA.24833.1.S1_s_at

++++

5

0.088

RPS14

LOC697734/LOC710901

MmugDNA.3842.1.S1_s_at

++++

6

0.091

RUVBL2

---

MmugDNA.33967.1.S1_at

+

7

0.092

EIF4A2

EIF4A2

MmugDNA.25666.1.S1_at

+++

8

0.093

RRAGA

BRI3

MmugDNA.15747.1.S1_at

+++

9

0.096

NDUFA1

NDUFA1

MmugDNA.13559.1.S1_at

+++

10

0.099

SNRPA

SNRPA

MmugDNA.6187.1.S1_at

+

11

0.100

PFDN1

LOC697795

MmugDNA.2620.1.S1_at

+++

12

0.100

PRPF8

PRPF8

MmugDNA.21692.1.S1_at

++

13

0.102

EMR2

EMR2

MmuSTS.3377.1.S1_at

+

14

0.104

similar to calmodulin

LOC715270/LOC717686

MmunewRS.652.1.S1_at

++++

15

0.105

CCND3

CCND3

MmuSTS.1979.1.S1_at

+

16

0.106

PSMD2

LOC712473

MmuSTS.2326.1.S1_at

+++

17

0.106

similar to ACTG1

LOC713687

Mmu.5709.1.S1_x_at

++++

18

0.106

UQCRQ

UQCRQ

MmugDNA.18513.1.S1_s_at

+++

19

0.106

NUDT3

LOC718568

MmugDNA.22176.1.S1_at

++

20

0.107

ARL2

LOC722013

MmugDNA.11257.1.S1_s_at

+++

HRT: treatment

rank/824

stability value

descriptor "GEN = "

gene symbol

probe set ID

exp

1

0.059

SUI1

---

MmugDNA.22897.1.S1_s_at

+++

2

0.063

RUVBL2

---

MmugDNA.33967.1.S1_at

+

3

0.068

TMED9

LOC702446

MmugDNA.32101.1.S1_s_at

++

4

0.071

PSMD2

LOC712473

MmuSTS.2326.1.S1_at

+++

5

0.072

SARS

LOC695046

MmugDNA.33979.1.S1_at

+++

6

0.073

AHSA1

AHSA1

MmugDNA.13760.1.S1_at

+++

7

0.074

RAD23A

LOC720675

MmugDNA.4491.1.S1_s_at

++

8

0.074

TCFL1

VPS72

MmuSTS.798.1.S1_at

+

9

0.074

TALDO1

LOC720828

MmugDNA.14258.1.S1_s_at

+++

10

0.075

NDUFA7

LOC711212

MmugDNA.17832.1.S1_at

++

11

0.077

RRAGA

BRI3

MmugDNA.15747.1.S1_at

+++

12

0.077

RPS14

LOC697734///LOC710901

MmugDNA.3842.1.S1_s_at

++++

13

0.077

AKR1A1

AKR1A1

MmugDNA.35875.1.S1_at

++

14

0.077

CYB5R1

LOC704519

MmuSTS.4187.1.S1_at

++

15

0.077

COX7A2L

LOC707203

MmugDNA.24749.1.S1_at

+

16

0.077

COX7A2L

LOC707203

MmugDNA.24749.1.S1_at

+

17

0.078

UBB

LOC697557

MmugDNA.35404.1.S1_at

++++

18

0.078

NDUFS5

NDUFS5

MmugDNA.29879.1.S1_at

+++

19

0.079

COX4I1

LOC714951

MmugDNA.39322.1.S1_s_at

+++

20

0.080

PFDN1

LOC697795

MmugDNA.2620.1.S1_at

+++

  1. 824 probe sets were selected from the 1444 HRT probe sets annotated for housekeeping genes based on MAS 5.0 signal intensity and detection call metrics. Probe sets in the table are in order of most stably expressed to least stably expressed within the top 20. "descriptor" is the gene symbol most closely representing annotation at the time of table creation. Here "GEN = " annotations were prioritized. "---" = no gene symbol listed. "exp" = gene expression represented by hybridization signal intensity [52].
  2. "+" and "-" symbols represent signal intensity values averaged across all animals and obtained using Affymetrix® GCOS analysis, and global scaling to an average target intensity of 200. (-) = "undetectable", (+) = "signal intensity < 500", (++) = "signal intensity 500-1500", (+++) = "signal intensity 1501-10000". (++++) = "signal intensity > 10000".
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BMC Molecular Biology

ISSN: 1471-2199

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