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Table 4 20 most stably expressed probe sets across brain regions of macaques in HRT study

From: Microarray analysis of relative gene expression stability for selection of internal reference genes in the rhesus macaque brain

HRT: brain region

rank/1444

stability value

descriptor "GEN = "

gene symbol

probe set ID

exp

1

0.064

SUI1

---

MmugDNA.22897.1.S1_s_at

+++

2

0.066

VEGFB

LOC722115

MmugDNA.8259.1.S1_at

-

3

0.073

1?

---

MmugDNA.15979.1.S1_at

-

4

0.073

RPS14

LOC697734

MmugDNA.3842.1.S1_at

-

5

0.074

SDC3

LOC704374

MmugDNA.32966.1.S1_at

-

6

0.074

RPS27A/ubiquitin

LOC697620/LOC701770

MmugDNA.43194.1.S1_at

-

7

0.074

2?

LOC693803

MmugDNA.23776.1.S1_at

-

8

0.076

MAPKAPK2

MAPKAPK2

MmugDNA.37463.1.S1_at

-

9

0.076

RPS14

LOC697734/LOC710901

MmugDNA.3842.1.S1_s_at

++++

10

0.076

TADA3L

TADA3L

MmugDNA.35773.1.S1_at

-

11

0.078

ACVRL1

ACVRL1

MmugDNA.35470.1.S1_at

-

12

0.078

CALM1

---

MmugDNA.30276.1.S1_s_at

-

13

0.079

STK24

---

MmugDNA.42176.1.S1_at

-

14

0.080

GPAA1

GPAA1

MmugDNA.10539.1.S1_s_at

+

15

0.080

COL6A1

---

MmugDNA.15324.1.S1_at

-

16

0.080

EEF1A1

LOC702809/LOC703715/LOC704199/LOC704438/LOC709017/LOC715351

MmuSTS.4277.1.S1_at

-

17

0.080

HIST1H2BC

LOC696506

MmunewRS.953.1.S1_x_at

-

18

0.080

COX7C

LOC693275/LOC702058/LOC706698

MmugDNA.43583.1.S1_x_at

+

19

0.081

RAD9A

LOC712345

MmugDNA.26624.1.S1_at

-

20

0.081

API5

API5

MmugDNA.34847.1.S1_at

-

HRT: treatment

rank/1444

stability value

descriptor "GEN = "

gene symbol

probe set ID

exp

1

0.053

ARHGEF7

ARHGEF7

MmugDNA.4544.1.S1_at

-

2

0.054

RPS27A/ubiquitin

LOC697620/LOC701770

MmugDNA.43194.1.S1_at

-

3

0.056

API5

API5

MmugDNA.34847.1.S1_at

-

4

0.057

HPCAL1

---

MmugDNA.15147.1.S1_at

-

5

0.058

GRIK5

---

MmugDNA.24126.1.S1_at

-

6

0.059

RPS25

LOC719947

MmugDNA.41046.1.S1_at

-

7

0.062

PFDN1

LOC697795

MmugDNA.2620.1.S1_at

+++

8

0.062

ATP5S

LOC710290

MmugDNA.29002.1.S1_at

-

9

0.062

EEF1A1

LOC702809/LOC703715/LOC704199/LOC704438/LOC709017/LOC715351

MmuSTS.4277.1.S1_at

-

10

0.062

FLJ10808

LOC711751

MmugDNA.6835.1.S1_at

-

11

0.063

GPAA1

GPAA1

MmugDNA.10539.1.S1_s_at

+

12

0.063

HSP?

LOC708240/LOC709412

Mmu.13079.1.S1_at

-

13

0.064

MCM3APAS

---

MmugDNA.7848.1.S1_at

-

14

0.064

RPL37

---

MmugDNA.8209.1.S1_at

-

15

0.064

dJ612B15.1

LOC698967

MmunewRS.398.1.S1_at

++++

16

0.064

SARS

---

MmugDNA.37510.1.S1_at

-

17

0.064

YWHAE

LOC720295

MmugDNA.14868.1.S1_at

-

18

0.064

RPS24

LOC701477/LOC702961/LOC704054/LOC705596/LOC707085/LOC711145/LOC715668/LOC717801

MmugDNA.6998.1.S1_at

++++

19

0.064

HIST1H2BC

LOC696506

MmunewRS.953.1.S1_at

-

20

0.064

ATP6V1F

LOC705175

MmugDNA.5612.1.S1_at

-

  1. 1444 probe sets annotated for genes in the independently verified set were analyzed. Probe sets in the table are in order of most stably expressed to least stably expressed within the top 20. "descriptor" is the gene symbol most closely representing annotation at the time of table creation. Here "GEN = " annotations were prioritized. "---" = no gene symbol listed. "exp" = gene expression represented by hybridization signal intensity [52].
  2. "+" and "-" symbols represent signal intensity values averaged across all animals and obtained using Affymetrix® GCOS analysis, and global scaling to an average target intensity of 200. (-) = "undetectable", (+) = "signal intensity < 500", (++) = "signal intensity 500-1500", (+++) = "signal intensity 1501-10000". (++++) = "signal intensity > 10000".
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BMC Molecular Biology

ISSN: 1471-2199

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