Microarray analysis of relative gene expression stability for selection of internal reference genes in the rhesus macaque brain

Table 1 Primer and TAMRA probe sequence information for qRT-PCR

Gene name	Primer & probe sequences (5' to 3')		Reference sequence	Amplicon length
	F-primer	AACAGTGCCACAGAGCGAGAA
ALG9	R-primer	CGATACCGCCTGGAGCACTA	XM_001106042	100
	probe	ACTGTCTTCCTGTTCGGG
	F-primer	AAGGGCATCCTGGGCTACA
GAPDH	R-primer	GAAGAGTGGGTGTCGCTGTTG	XM_001105471	66
	probe	TGAGCACCAGTGGTCTCCTCCGACT
	F-primer	TCACGAGGTTGGCTGGAAGT
RPL13A	R-primer	GATCTTGGCTTTCTCCTTCCTCTT	XM_001115079	72
	probe	CCAGGCAGTGACAGCCACCTTGG
	F-primer	GGAAAATACACCGTGTTTTCCTAAA
GABRA1	R-primer	AGGCAGGACCAAATCAAACAA	BV208518	72
	probe	ACACCTTTCTTTTTACATGTGCTTC
	F-primer	TCCCTCTCTGCGTGTTTCAA
GABRD	R-primer	GCCGAGGCTTCCTCTTGTTT	MMUGDNA.30046.1.S1_AT *	63
	probe	TGGGATGACAGTCGGCCACGG
	F-primer	GGCACTGGAACTTTGGCAAA
GABRE	R-primer	TGTCACAGGGCTATCATGAAGCT	BV166270	70
	probe	CACCTTTGACAAATTGTGTCTATTTG
	F-primer	CTCCTCAACTATGTCCGCAAGA
GAD1	R-primer	TCCAAGTTGAAGCCCTCCAT	NM_013445	100
	probe	TTCCATCACCCACACCAGTTGCTGG
	F-primer	CTCGAAAGGCTTCAATTGCAT
GABRA4	R-primer	CTTCCCAGTAGCCCCTATGGT	NM_000809	100
	probe	TGCAATCTTGATCCAAACACGTGACGA
	F-primer	CACCATTGCCCGGAAATC
GABRG2	R-primer	ACTCCACCAAAGCAGAGAAGACA	NM_198904	100
	probe	TCACAGCGATGGATCTCTTTGTATC

F = forward, R = reverse. Reference sequence = NCBI accession except for GABRD.
* = Probe set sequence using NetAffx annotation for the GeneChip^® rhesus Macaque Genome Array http://www.affymetrix.com/analysis/index.affx

ISSN: 1471-2199