Figure 2

Relative gene expression stability in the rhesus macaque brain. GeNorm graphical output showing relative stability of genes expressed in three brain areas from three hormone replacement regimes. Stability values (M) are based on pairwise comparisons between the gene of choice and all other genes under consideration. The chart shows final stability values resulting from sequential elimination of the least stable genes after each set of pairwise analyses [34]. Therefore, the ordinate value shown for each gene represents the M-value obtained from the iteration before the gene was eliminated from analysis. Elimination sequence is shown from left to right. More stably expressed genes have lower M scores. Here the sample responsible for greatest influence on MBH variation was omitted from the analysis. When this sample was included the rank order of GAPDH and GAD1 were reversed.