Figure 1

Study methodology schematic. Flowchart outlining organization and groupings for analyses of data from the HRT and cycle studies. Alternating black and red text color is used for association of headings (left column) with appropriate rows of flowchart boxes.

The six GABAergic pathway genes and three predicted normalizers for qRT-PCR are detailed in the methods. Numbered designations (1a, 1b... 2c) refer to separate selections of sequences to be analyzed (detailed in methods), with HRT sequences from 1a to 1c, and cycle study sequences from 2a to 2c. AMD = amygdala; E₂ = 17β-estradiol; E₂+P₄ = 17β-estradiol + progesterone; EF = early follicular (menstrual cycle phase); GABA = gamma-aminobutyric acid; HPC = hippocampus; LF = late follicular (menstrual cycle phase); MBH = arcuate nucleus of the medial basal hypothalamus; ML = mid luteal (menstrual cycle phase); OVX = ovariectomized; qRT-PCR = quantitative real-time polymerase chain reaction;