Table 1 Primers used for RT-PCR analysis of Enah transcripts with or without exons 3a, 3b, 6L and/or 11a.
Transcript with (+) or without (-) alternative exons: | Primer namea | Sequence |
|---|---|---|
+ 3a and + 3b | Fw 3a_3b | 5'-GCGATGTATTTTCTGTGTGTTC-3' |
+ 3a and - 3b | Fw 3a_4 | 5'-GCGATGTATTTTCTGTGGGC-3' |
- 3a and + 3b | Fw 3_3b: | 5'-ATTCACAGGAAGCAGTGTTC-3' |
- 3a and - 3b | Fw 3_4 | 5'-TTCACAGGAAGCAGGGC-3' |
+ 6L | Rev 6L_5 | 5'-GTCTGAAGATGGAGCAGC-3' |
Rev 7_6 | 5'-GTCCCAAGACAAGGCCC-3' | |
Fw 6L_6 | 5'-TTCTCCCTCTGCAGCTGC-3' | |
-6L | Rev 7_6 | 5'-GTCCCAAGACAAGGCCC-3' |
Fw 5_6 | 5'-AGAATGTCCAATGCTGCTG-3' | |
+11a | Rev 12_11a | 5'-GTGTGGATTTGGGTCTGTG-3' |
-11a | Rev 12_11 | 5'-TGTGGATTTGGGTCTGGAG-3' |