Figure 4
Expression of mouse Enah transcripts with exon 6. A. Details of the RT-PCR reactions: the primers used, the presence (+) or absence (-) of alternative exons and intron and the expected length of the amplicon in base pairs (bp) are indicated. NA, not applicable. B-C-D. The numbers 1-6 of the RT-PCR reactions on the left refer to the primer combinations in panel A. The two bottom panels are the amplifications of the household genes glyceraldehyde-3-phosphate dehydrogenase (Gapdh) and 18S ribosomal RNA (Rn18s). The molecular weight markers (in base pairs) are indicated on the left. The expected size of the amplicon in base pairs is on the right side of the panels. B. Expression of transcripts in whole mouse embryos of day E9.5 to E18.5 and in head and body fractions of E16.5 to E18.5. C. Expression of transcripts in adult mouse tissues. D. Expression of transcripts in the indicated mouse cell lines. MVD7 cells are derived from the Mena/VASP double knockout mouse [27].