Table 1 Sequences of primers.
Primer | Sequence (5'→ 3') | Nucleotide position |
|---|---|---|
1S | TACCTGTGTCTGCCTGCTG | 1910-1928 in βc |
1A | GAGACATAACCAGAGGCCACT | 2136-2166 in βc |
2S | TGCCAGAGTTTTGTCGTCAC | 326-345 in βc |
2A | CTTGCTGGGACGTCCTGAGA | 692-712 in βc |
3S | CTTGGGAGGTAGGAACCACG | 570-(i)12* in βc |
HygRS | TGGATATGTCCTGCGGGTAA | 2073-2092 in IREShyg2 |
HygRA | TTCCTTGCGGTCCGAATG | 2345-2362 in IREShyg2 |
EpoRS | CCACTGCTTACTGGCTTATCG | 838-859 in IREShyg2 |
EpoRA | TGCTCTCAAACTTGGGGTCCG | 119-139 in EpoR |