Figure 3

Dose-response relationship between βc79 transcripts and translation inhibitors. The βc79-expressing clone mE was incubated with various concentrations of puromycin, emetin, and CHX for 4.5 hours. The ratios of βc-specific transcripts to GAPDH transcripts were measured by real-time RT-PCR with 1S/1A primers. The ratios in NMD inhibitor-treated cells were divided by those in untreated cells, which are presented as fold increases. The mean values ± S.E.M. (error bars) were obtained from 3 to 4 independent experiments.