Figure 3
From: Halogenated imidazole derivatives block RNA polymerase II elongation along mitogen inducible genes
TBBz and DMAT arrest RNAPII elongation along the EGR1 locus after serum treatment. (A) Position of primers relative to transcription start sites (in bp): exon 1 +248; exon 2 +1287; exon 2.1 +3490; 1 kb +4528. (B, C) HeLa cells maintained for 48 h in 0.5% serum were treated with fresh medium supplemented with 15% FBS and -/+ 25 μM TBBz/50 μM DRB/10 or 25 μM DMAT (C) for the indicated times and then used in ChIP assays with antibodies to RNAPII. Purified DNA was used in real-time PCR with pairs of primers spanning the EGR1 locus. The density of RNAPII on the EGR1 gene was quantified by real-time PCR and is presented as a percentage of input. Data represent means ± S.D from 3 independent experiments.