Figure 5From: The transforming acidic coiled coil (TACC1) protein modulates the transcriptional activity of the nuclear receptors TR and RARTACC1 participates to the modulation of transcriptional activity by nuclear receptors. (A) The protein levels of TACC1 in control (non-targeting siRNA) or TACC1 siRNA treated cells were determined by immunoblotting with an anti-TACC1 antibody (Upstate). β-tubulin was used as a loading control. (B, C, D, E) Non-targeting siRNA or TACC1 siRNA treated HEK-293F cells were transiently transfected with TRE-TKLuc and TRα1 cDNA expression vector (B), RARE-TKLuc (C), Oct4-Binding Domain-Luc and Oct4 and Sox2 expression plasmids (D) or 5xcoll TATA Luc (E). Cells were treated with 10-7M of T3 (B) or all-trans RA (ATRA) (C) for 48 h. Firefly luciferase was normalized for cotransfected Renilla luciferase activity (RLU). Open histogram, control siRNA treated cells; black histogram, TACC1 siRNA treated cells. The bars indicate the mean ± S.E. of triplicate transfections from a representative experiment (* p < 0.05, ** p < 0.01, by Student's-test).Back to article page