Figure 4From: The transforming acidic coiled coil (TACC1) protein modulates the transcriptional activity of the nuclear receptors TR and RARTACC1 interacts with RARα in the nucleus of F9 cells and is present in a nuclear chromatin-enriched fraction. (A) Cells from an F9 cell line stably expressing Flag-RARα were fractionated into cytoplasmic (C) and nuclear (N) fractions. 800 μg of total protein of each fraction were immunoprecipitated with an anti-Flag antibody. Immunoprecipitates were resolved by SDS-PAGE and blotted with anti-Flag or anti-TACC1antibodies, which reveal only the longest TACC1 proteins (A and K). Absence of cross contamination between cytosolic and nuclear fractions was verified using respectively anti-β-tubulin and anti-Histone H3 antibodies. The same amount of protein was loaded in each lane. (B) F9 cells were fractionated into cytosolic (lane 1), soluble nuclear (lane 2) and chromatin-enriched (lane 3) fractions. Cell-equivalent amounts of fractions were probed by immunoblotting with anti-TACC1, anti-RARα, and anti-Histone H3 antibodies.Back to article page