From: Reference gene alternatives to Gapdh in rodent and human heart failure gene expression studies
Gene Symbol | Genbank ID | Assay information | Primer location on Refseq | Amplicon (bp) | Target exons | E |
---|---|---|---|---|---|---|
Mouse genes | ||||||
Rplp0 (Arbp) | NM_007475 | AB ID Mm00725448_s1 | 124 | 7 | 0.86 | |
Rpl32 | NM_172086 | F: CAC CAG TCA GAC CGA TAT GTG AAA A | 118-142 | 64 | 2-3 | 0.92 |
R: TGT TGT CAA TGC CTC TGG GTT T | 160-181 | |||||
P: CCG CCA GTT TCG CTT AA | 143-159 | |||||
Rpl4 | NM_024212 | F: GGT GGT TGA AGA TAA GGT TGA AGG T | 500-524 | 71 | 5 | 0.88 |
R: CTT TGA GTT TCT TGA GCA GCT GAA | 547-570 | |||||
P: CAG CCT CCT TGG TCT TC | 530-546 | |||||
Tbp | NM_013684 | AB ID Mm00446973_m1 | 73 | 4-5 | 0.78 | |
Polr2a | NM_009089 | F: CTT TGA GGA AAC GGT GGA TGT C | 4293-4314 | 67 | 26 | 0.94 |
R: TCC CTT CAT CGG GTC ACT CT | 4340-4359 | |||||
P: ATG TGC TGC TGC TTC C | 4320-4335 | |||||
Hprt1 | NM_013556 | AB ID Mm00446968_m1 | 65 | 6-7 | 0.84 | |
Gapdh | NM_008084 | AB ID Mm99999915_g1 | 107 | 2-3 | 0.79 | |
Pgk1 | NM_008828 | AB ID Mm00435617_m1 | 137 | 5-6 | 0.77 | |
Ppia | NM_008907 | F: GCA CTG CCA AGA CTG AAT GG | 385-404 | 63 | 4-5 | 0.81 |
R: TGC CTT CTT TCA CCT TCC CAA A | 426-447 | |||||
P: CTG GAT GGC AAG CAT G | 405-420 | |||||
Atp2a2 | NM_009722 | AB ID Mm00437634_m1 | 59 | 20-21 | 0.87 | |
Pln | NM_023129 | AB ID Mm00452263_m1 | 71 | 1-2 | 0.83 | |
Slc8a1 (Ncx1) | NM_011406 | AB ID Mm00441524_m1 | 72 | 10-11 | 0.78 | |
Nppa | NM_008725 | F: GTA CAG TGC GGT GTC CAA CA | 170-189 | 1-2 | 0.80 | |
R: CTC ATC TTC TAC CGG CAT CTT CTC | 231-254 | |||||
P: AAG AAC CTG CTA GAC CAC C | 207-225 | |||||
Rat genes | ||||||
Arbp (Rplp0) | NM_022402 | AB ID Rn00821065_g1 | 97 | 1-2 | 0.90 | |
Rpl32 | NM_013226 | AB ID Rn00820748_g1 | 72 | 1 | 0.80 | |
Rpl4 | NM_022510 | AB ID Rn00821091_g1 | 82 | 5-6 | 0.84 | |
Tbp | NM_001004198 | F: CCT CTG AGA GCT CTG GGA TTG TA | 666-688 | 62 | 3-4 | 1.10 |
R: GCC AAG ATT CAC GGT GGA TAC A | 706-727 | |||||
P: CCA CAG CTC CAA AAT A | 689-704 | |||||
Polr2a | XM_343922 | F: CGT ATC CGC ATC ATG AAC AGT GA | 4181-4203 | 71 | 22-24 | 0.92 |
R: TCA TCC ATC TTA TCC ACC ACC TCT T | 4227-4251 | |||||
P: CCT CCT CCT GCA TCT TG | 4210-4226 | |||||
Hprt1 | NM_012583 | AB ID Rn01527838_g1 | 100 | 4-5 | 0.86 | |
5-HTT (Slc6a4) | NM_013034 | F: GTC ATC TGC ATC CCT ACC TAT ATC ATT | 1863-1889 | 98 | 15-16 | 0.76 |
R: GTG GGT GTT TCA GGA GTG ATA CTT T | 1936-1960 | |||||
P: AAT AAT CCG CTC CTT AAG TGT CCC CGG AGT | 1905-1934 | |||||
Gapdh | NM_017008 | AB ID Rn99999916_s1 | 87 | 3 | 0.93 | |
Atp2a2 | NM_017290 | AB ID Rn00568762_m1 | 83 | 3-4 | 0.91 | |
Pln | NM_022707 | AB ID Rn01434045_m1 | 59 | 1-2 | 0,87 | |
Slc8a1 (Ncx1) | NM_019268 | AB ID Rn00570527_m1 | 115 | 5-6 | 0,88 | |
Nppa | NM_012612 | AB ID Rn00561661_m1 | 58 | 2-3 | 0.96 | |
Human genes | ||||||
Rplp0 (Arbp) | NM_001002 | AB ID Hs99999902_m1 | 105 | 3 | 0.85 | |
Rpl32 | NM_000994 | F: CAC CAG TCA GAC CGA TAT GTC AAA A | 161-185 | 64 | 2-3 | 0.93 |
R: TGT TGT CAA TGC CTC TGG GTT T | 203-224 | |||||
P: CCG CCA GTT ACG CTT AA | 186-202 | |||||
Rpl4 | NM_000968 | F: CAG CAC TGG TCA TGT CTA AAG GT | 457-479 | 81 | 4-5 | 0.93 |
R: AGC CTT CAA CTT TAT CTT CAA CTA CCA AA | 537-509 | |||||
P: CAT CGT ATT GAG GAA GTT C | 480-498 | |||||
Tbp | NM_003194 | AB ID Hs00427620_m1 | 91 | 3-4 | 0.97 | |
Polr2a | NM_000937 | AB ID Hs00172187_m1 | 61 | 1-2 | 0.87 | |
Hprt1 | NM_000194 | AB ID Hs00355752_m1 | N/A | N/A | 0.74 | |
Gapdh | NM_002046 | AB ID Hs99999905_m1 | 122 | 3 | 0.98 | |
Pgk1 | NM_000291 | AB ID Hs99999906_m1 | 75 | 4-5 | 0.74 | |
Ppia | NM_021130 | AB ID Hs99999904_m1 | 98 | 4 | 0.75 | |
Atp2a2 | NM_001681 | AB ID Hs00544877_m1 | 123 | 5-6 | 0.90 | |
Pln | NM_002667 | AB ID Hs00160179_m1 | 98 | 1-2 | 0.69 | |
Slc8a1 (Ncx1) | NM_021097 | AB ID Hs00253432_m1 | 58 | 2-3 | 0.83 | |
Nppa | NM_006172 | AB ID Hs00383230_g1 | 105 | 1-2 | 0.81 | |
18S | X03205 | Eukaryotic 18S endogenous control, part number 4319413E | 187 | N/A | 1.06 |