Figure 4

Phosphorylation of H2AX associated with cell cycle progression and the relative contributions of DNA-PKcs. A: Levels of γH2AX associated with cell cycle progression in DNA-PKcs-depleted HeLa-H1 (right) and control HeLa-NC cells (left). B: The effect of wortmannin on cell-cycle associated H2AX phosphorylation. After G1 synchronization by TdR double-blocking, cells were cultured in fresh DMEM supplemented with 2 μM wortmannin, then collected at 0, 5, 8, 9, 10 h, and analyzed by Western blotting. C: The effect of NU2076 on cell-cycle associated H2AX phosphorylation. After G1 synchronization by TdR double-blocking, cells were cultured in fresh DMEM supplemented with 10 μM NU2076, then collected at 0, 5, 8, 9, 10 h, and analyzed by Western blotting.