Hfq affects mRNA levels independently of degradation

BMC Molecular Biology

Table 2 Escherichia coli strains and plasmids used in this work

Strain or plasmid	Relevant characteristics	Reference/source
Strains
N3433	HfrHlacZ43 relA1 spoT1 thi-1	D. Apirion
N3431	N3433 rne3071(ts)	D. Apirion
IBPC633	N3433 rnc105	[34]
IBPC903	N3433 ΔpcnB(kan^R)	[58]
IBPC927	IBPC633 hfq1::Ω(kan^R, Bcl I)	this work
IBPC928	N3431 hfq1::Ω(kan^R, Bcl I)	this work
IBPC929	N3433 hfq1::Ω(kan^R, Bcl I)	[16]
IBPC941	N3433 hfq V43R cycA30::Tn10	[16]
IBPC953	N3433 hfq Δ22-294 cycA30::Tn10	[16]
IBPC981	IBPC903 hfq Δ22-294 cycA30::Tn10	this work
ENSO	HfrG6lacZ Δ12	[56]
IBrpsO188::lacZ(*)	ENSO rpsO'-'lacZ	this work
LABrpsB208ΔGGGU(*)	ENSO rpsB'-'lacZ bearing deletion in
	rpsB TIR from -72 to -69	[43]
Plasmids
pΔS15AUG	pCL1921 derivative expressing ΔrpsO	[38]
pACYC184	Tet^r, Cm^r	[59]
pS15	pACYC184 derivative expressing rpsO	this work
pGEM3	Amp^r	Promega corp.
pTX367 (**)	pGEM3 derivative expressing hfq	[5]
pTX381 (**)	pACYC184 derivative expressing hfq	[5]

(*) Tetracyclin-resistant derivatives of these strains, bearing cycA30::Tn10 and hfq Δ22-294 cycA30::Tn10, were constructed for using in this work. (**) These two plasmids contain the same insert. They were used without distinction assuming that the same amount of Hfq is overproduced due to autoregulation [16, 17].

ISSN: 1471-2199