Figure 1From: The Renilla luciferase gene as a reference gene for normalization of gene expression in transiently transfected cellsThe effect of an increasing transfection concentration of pRL-SV40 DNA, a control plasmid encoding Rluc , on Rluc gene expression. Rluc expression was normalized to ActB gene expression. Each sample represents the expression of Rluc/ActB in a total RNA concentration of 0.2 μg.Back to article page